The studies were undertaken to determine the effects of nephrectomy, intestinal rese￢ction and portocaval shunt on the plasma gastrin responses to casein hydrolysate using 45 Spragle-Dawley rats weighing 170 to 2?J.,m each. The rats were consisted of 10 normal, 10 sham operated control, 10 functionally nephrectomized, 10 functionally resected small bowel and 5 with portocaval shunt.
One gram of casein hydrolysate per 200 gm body weight was administered orally to the rats at the fasting state.
Blood samples were drawn from the internal orbital venous plexus for 60 minutes after casein administration and plasma gastrin concentrations were measured by the radioimmunoassay.
Followings were the results
1. Administration of large amount of casein hydrolysate(lgm) to normal rat resulted in a progressively increased plasma gastrin concentration in course of time by 60 minutes.
2. Plasma gastrin response in the rats with sham operation was higher than that of normal rats.
3. In the nephrectomized group, significantly elevated plasma gastrin response was shown, especially after 43 minutes following casein administration.
4. In the small bowel rejected group, elevated plasma gastrin response was also shown, while the level was slightly lower than that of nephrectomized group.
5. In the portocaval shunt group, plasma gastrin response was shown initial marked increase and followed by elevated range during the course of time.
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